Estriol ELISA Kit from Innovative Research is intended for the quantitative determination of Estriol in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a competitive inhibition ELISA in strip well format with reagents for up to 96 tests . E3 present in samples reacts with an anti-E3 monoclonal capture antibody or antigen coated and dried on a microtiter plate. Following this, E3 conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active E3 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of E3. Detection Target: Estriol Reactivity: Multi-species Range: 1.56-100ng/mL Sensitivity: 0.52ng/mL Storage Conditions: Store all components at 4┬░C This Multi-species E3 activity assay is for the quantitative determination of Estriol in samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.