Human Alpha-Endorphin ELISA Kit from Innovative Research is intended for the quantitative determination of Human Alpha-Endorphin in biofluid samples, such as serum, plasma, tissue homogenates, cell culture supernatants and other biological fluid. This is a competitive inhibition ELISA in strip well format with reagents for up to 96 tests . aEP present in samples reacts with an anti-Human aEP monoclonal capture antibody or antigen coated and dried on a microtiter plate. Following this, aEP conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active aEP in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human aEP. Detection Target: Human Alpha-Endorphin Reactivity: Human Range: 15.6-1000pg/mL Sensitivity: 4.45pg/mL Storage Conditions: Store all components at 4┬░C This Human aEP activity assay is for the quantitative determination of Alpha-Endorphin in samples including serum, plasma, tissue homogenates, cell culture supernatants and other biological fluid. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.