Human Casein Beta ELISA Kit from Innovative Research is intended for the quantitative determination of Human Casein Beta in biofluid samples, such as breast milk and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . CSN2 present in samples reacts with an anti-Human CSN2 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-CSN2 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active CSN2 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human CSN2. Detection Target: Human Casein Beta Reactivity: Human Range: 78.1-5000ng/mL Sensitivity: 27ng/mL Storage Conditions: Store all components at 4┬░C This Human CSN2 activity assay is for the quantitative determination of Casein Beta in samples including breast milk and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.